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Rat bFGF ELISA Kit
来自 : 发布时间:2024-05-14
货号: 3030037
样本: Serum, plasma, Cell culture supernatant
标记物: Rat
适应物种: Rat
应用: 详见说明书
检测方法: 详见说明书
检测限: 2.05-500pg/ml
供应商: 索莱宝
数量: 电询
规格: 96 tests
英文名称英文别名中文别名ReactivitySample typeSensitivityDetection RangeAssay timeDetection TargetApplicationsSwissProtIDNCBI Full Gene NameNCBI Gene AliasesProduct SummaryBackground
Rat bFGF ELISA Kit
Rat bFGF ELISA Kit,fibroblast growth factor 2,Fgf-2, bFGF
大鼠碱性成纤维细胞生长因子ELISA试剂盒
Rat
Serum, plasma, Cell culture supernatant
2pg/ml
2.05-500pg/ml
4.5h
bFGF
Sandwich ELISA
P13109
fibroblast growth factor 2
Fgf-2, bFGF
The Bioaim Rat bFGF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of bFGF in rat serum, plasma and cell culture media. An antibody specific for rat bFGF was coated on a 96-well plate. Standards and samples are added to the wells and any bFGF present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat bFGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of bFGF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
温馨提示:不可用于临床治疗。

本文链接: http://bioaims.immuno-online.com/view-1521543739.html

发布于 : 2024-05-14 阅读()
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\研究课题\ ELISA 抗体
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