Product Features
Product Name Human CD26 EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity 80pg/mL Assay Range 0.49-120ng/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names DPP4; ADABP; ADCP2; CD26; DPPIV; TP103 Gene ID 1803 Swissprot ID P27487
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 84 | 73-90 |
| Plasma | 92 | 88-94 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 74 |
| 1:4 | 81 | |
| 1:8 | 73 | |
| Plasma | 1:2 | 83 |
| 1:4 | 87 | |
| 1:8 | 82 |
Standard Curve: Human CD26 EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human CD26 mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
The protein, encoded by the DPP4 gene is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
It is a rather indiscriminate enzyme for which a diverse range of substrates are known. The substrates of CD26/DPPIV are proline(or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human CD26 EasyTest™ ELISA Kit is for Research Use Only.
