Product Name | GSK3a(Phospho-Ser21) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Applications | WB IHC IF |
Reactivity | Hu Ms Rt |
Specificity | The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9. |
Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a. |
Target Name | GSK3a |
Alternative Name | Factor A; GSK-3 alpha; kinase GSK3-alpha |
Modification | Phospho-Ser21 |
SDS-PAGE MW | 51kd |
Accession No | P49840; NP_063937.2 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use. |
Application:
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Background
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760. Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.