BioaimScientific/PTEN（磷酸化Ser380）抗体/100ul/11009
市场价：
¥5580.00
美元价：
2790.00
产品分类：
细胞成像系统
公司分类：
Cell_imaging_system
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Name | PTEN(Phospho-Ser380) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Applications | IHC IF |
Reactivity | Hu |
Immunogen | Peptide-KLH |
Target Name | PTEN |
Alternative Name | BZS, DEC, GLM2, MHAM, TEP1. |
Modification | Phospho-Ser380 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use. |
Background
Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability.
Li D.M., Sun H. Cancer Res. 57:2124-2129(1997) Song M.S., Salmena L., Carracedo A., Egia A., Lo-Coco F., Teruya-Feldstein J., Pandolfi P.P.Nature 455:813-817(2008)
Myers M.P., Stolarov J.P., Eng C., Li J., Wang S.I., Wigler M.H., Parsons R., Tonks N.K.Proc. Natl. Acad. Sci. U.S.A. 94:9052-9057(1997)
品牌介绍
BioaimScientific产品名称 人脂联素EasyTest™ELISA试剂盒测定类型 固相夹心ELISA格式 96孔条板测定过程 一次洗涤，两次温育测定长度 2.5小时样品体积 血清，血浆，50µL检测方法 比色灵敏度 18pg / mL测定范围 163.8- 40000pg / mL的反应性 人类交叉反应性 没有可用相关分子中观察到的交叉反应性基因名称 ADIPOQ; ACDC；ACRP30；ADIPQTL1; ADPN；APM-1；APM1；28英镑基因ID 9370Swissprot ID Q15848 复苏样品类型 平均回收率范围 ％血清 10294-105等离子体 10697-102线性度样品类型稀释预期百分比血清1：2981：41011：890等离子体1：2971：41021：889标准曲线：人脂联素EasyTest™ELISA试剂盒套件组件•预包被的96孔板微孔板•洗涤缓冲液•终止溶液•分析稀释液•标准品•Bioaim人类脂联素混合物•TMB一步底物协议大纲1.按照手册中的说明准备所有试剂，样品和标准品。2.向每个孔中添加50 µl标准液或样品。3.加入50 µl Bioaim混合物。4.在室温下孵育2小时。5.洗涤并向每个孔中添加100 µl TMB一步底物试剂。6.在室温下孵育15分钟。7.在每个孔中加入50 µl终止溶液。8.在450 nm / 570 nm处读取。背景脂联素（也称为30 kDa的脂肪细胞补体相关蛋白，Acrp30）是脂肪细胞衍生的蛋白，对代谢和炎症具有广泛的旁分泌和内分泌作用。它促进脂肪细胞分化，脂肪酸分解代谢和胰岛素敏感性，并且与肥胖症，2型糖尿病和动脉粥样硬化形成负相关。脂联素专门从脂肪组织分泌到血液中，相对于许多激素，血浆中的脂联素非常丰富。成年人体内的激素水平与体内脂肪百分比成反比。同样，动物和人类（例如神经性厌食症）的热量限制期间，循环脂联素浓度会增加。然而，最近的一项研究表明，在热量限制期间增加的骨髓内脂肪组织在这种情况下会导致循环脂联素升高。相关产品人脂联素ELISA试剂盒存储从发货之日起，整个套件可在-20°C下最多保存1年。避免重复冻融循环。该试剂盒可在4°C下最多保存6个月。对于长时间存储，建议在-80°C下存储。监管状况 人脂联素EasyTest™ELISA试剂盒仅供研究使用。
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