Product Name | PDK1(Phospho-Ser241) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Applications | WB IHC IF |
Reactivity | Hu Ms Rt |
Specificity | The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241. |
Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1. |
Target Name | PDK1 |
Alternative Name | PDPK1; PkB kinase; Protein kinase B kinase; hPDK1; kinase PDK1. |
Modification | Phospho-Ser241 |
SDS-PAGE MW | 63kd |
Accession No | O15530; NP_002604.1 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use. |
Application:
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Background
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.
Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63 Chen H, et al. (2001) Biochemistry; 40(39): 11851-9 Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7 Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11