Product Features
Product Name Human bFGF EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity 25 pg/mL Assay Range 31.3-2000pg/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names BFGF; FGF-2; FGFB; HBGF-2 Gene ID 2247 Swissprot ID P09038
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 91 | 82-101 |
| Plasma | 94 | 85-103 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 98 |
| 1:4 | 92 | |
| 1:8 | 91 | |
| Plasma | 1:2 | 95 |
| 1:4 | 96 | |
| 1:8 | 92 |
Standard Curve: Human bFGF EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human bFGF mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. Its bioactivity is modulated by a number of other binding partners including heparin, FGF-BP, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, PDGF, and CXCL4/PF4. In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples.
bFGF has been shown in preliminary animal studies to protect the heart from injury associated with a heart attack, reducing tissue death and promoting improved function after reperfusion. Recent evidence has shown that low levels of FGF2 play a key role in the incidence of excessive anxiety.
Related Products
Human bFGF ELISA Kit
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human bFGF EasyTest™ ELISA Kit is for Research Use Only.
