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BioaimScientific/人VEGF R3 EasyTest™ ELISA试剂盒/5-板试剂盒/1010017
Product Features
Product Name Human VEGF R3 EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity 15 pg/mL Assay Range 28.7.-7000 pg/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names FLT4; FLT-41; LMPH1A; PCL; VEGFR-3; VEGFR3 Gene ID 2324 Swissprot ID P35916
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 94 | 78-106 |
| Plasma | 109 | 107-111 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 91 |
| 1:4 | 73 | |
| 1:8 | 86 | |
| Plasma | 1:2 | 91 |
| 1:4 | 75 | |
| 1:8 | 82 |
Standard Curve: Human VEGF R3 EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human VEGF R3 mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.
Related Products
Human VEGF R1 ELISA Kit
Human VEGF R2 ELISA Kit
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human VEGF R3 EasyTest™ ELISA Kit is for Research Use Only.
