Product Features
Product Name Human IP-10 EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity < 1pg/mL Assay Range 41.9-10000pg/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names CXCL10; C7; IFI10; INP10; IP-10; SCYB10, crg-2, gIP-10, mob-1 Gene ID 3627 Swissprot ID P02778
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 105 | 83-117 |
| Plasma | 106 | 93-115 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 98 |
| 1:4 | 86 | |
| 1:8 | 80 | |
| Plasma | 1:2 | 98 |
| 1:4 | 99 | |
| 1:8 | 108 |
Standard Curve: Human IP-10 EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human IP-10 mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human IP-10 EasyTest™ ELISA Kit is for Research Use Only.
