Product Features
Product Name Human VEGF EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity 20 pg/pg/mL Assay Range 40.9-10000 pg/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names VEGFA; MVCD1; VEGF; VPF Gene ID 7422 Swissprot ID P15692
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 112 | 91-128 |
| Plasma | 97 | 91-108 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 130 |
| 1:4 | 141 | |
| 1:8 | 122 | |
| Plasma | 1:2 | 122 |
| 1:4 | 130 | |
| 1:8 | 123 |
Standard Curve: Human VEGF EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human VEGF mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis. In addition to binding to VEGFRs, VEGF binds to receptor complexes consisting of both neuropilins and VEGFRs. This receptor complex has increased VEGF signalling activity in endothelial cells. Neuropilins are pleitrophic receptors and therefore other molecules may interfere with the signalling of the NRP/VEGFR receptor complexes.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human VEGF EasyTest™ ELISA Kit is for Research Use Only.
