Product Features
Product Name Human Insulin EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity < 1 µIU/mL Assay Range 1.87-120µIU/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names INS; IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10 Gene ID 3630 Swissprot ID P01308
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 112 | 99-133 |
| Plasma | 101 | 98-103 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 126 |
| 1:4 | 103 | |
| 1:8 | 94 | |
| Plasma | 1:2 | 98 |
| 1:4 | 87 | |
| 1:8 | 95 |
Standard Curve: Human Insulin EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human Insulin mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
The human insulin protein is composed of 51 amino acids, and has a molecular weight of 5808 Da. Insulin is a peptide hormone produced by beta cells in the pancreas. It regulates the metabolism of carbohydrates and fats by promoting the absorption of glucose from the blood to skeletal muscles and fat tissue and by causing fat to be stored rather than used for energy. Insulin is provided within the body in a constant proportion to remove excess glucose from the blood. When blood glucose levels fall below a certain level, the body begins to use stored sugar as an energy source through glycogenolysis, which breaks down the glycogen stored in the liver and muscles into glucose, which can then be utilized as an energy source.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human Insulin EasyTest™ ELISA Kit is for Research Use Only.
