Product Features
Product Name Human BDNF EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 1.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity 10pg/mL Assay Range 93.8-6000pg/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names BDNF; ANON2; BULN2 Gene ID 627 Swissprot ID P23560
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 122 | 102-135 |
| Plasma | 115 | 109-123 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 114 |
| 1:4 | 113 | |
| 1:8 | 132 | |
| Plasma | 1:2 | 96 |
| 1:4 | 101 | |
| 1:8 | 110 |
Standard Curve: Human BDNF EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human BDNF mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 1 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
Related Products
Human BDNF ELISA Kit
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human BDNF EasyTest™ ELISA Kit is for Research Use Only.
