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BioaimScientific/Human Adiponectin EasyTest™ ELISA Kit/5-plate-kit/1010001
Product Features
Product Name Human Adiponectin EasyTest™ ELISA Kit Assay Type Solid Phase Sandwich ELISA Format 96-well strip plate Assay Process One wash step, two incubations Assay Length 2.5 hours Sample Volume Serum , Plasma, 50µL Detection Method Colorimetric Sensitivity 18pg/mL Assay Range 163.8-40000pg/mL Reactivity Human Cross-Reactivity No cross-reactivity observed with available related molecules Gene Names ADIPOQ; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28 Gene ID 9370 Swissprot ID Q15848
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 102 | 94-105 |
| Plasma | 106 | 97-102 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 98 |
| 1:4 | 101 | |
| 1:8 | 90 | |
| Plasma | 1:2 | 97 |
| 1:4 | 102 | |
| 1:8 | 89 |
Standard Curve: Human Adiponectin EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate • Wash Buffer • Stop Solution • Assay Diluent • Standards • Bioaim human Adiponectin mix • TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual. 2. Add 50 µl of standard or sample to each well. 3. Add 50 µl of Bioaim mix. 4. Incubate 2 h at RT. 5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well. 6. Incubate 15 min at RT. 7. Add 50 µl of Stop Solution to each well. 8. Read at 450 nm/570 nm.
Background
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones.
Levels of the hormone are inversely correlated with body fat percentage in adults; Similarly, circulating adiponectin concentrations increase during caloric restriction in animals and humans, such as in patients with anorexia nervosa. However, a recent study suggests that adipose tissue within bone marrow, which increases during caloric restriction, contributes to elevated circulating adiponectin in this context.
Related Products
Human Adiponectin ELISA Kit
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human Adiponectin EasyTest™ ELISA Kit is for Research Use Only.
